| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Rhizobium leguminosarum | TGCAGCAGGCCTTGTTCA | TCGATGCCCTTTTCCGCA | 59.80 | 59.65 | 250 | 47 |
100.00%
|
50.00%
|
| Primer 22 | Rhizobium leguminosarum | TTTCCTTCGCGTTGCCCT | CGCCTGCGAACAATGTCTG | 59.89 | 59.87 | 188 | 45 |
100.00%
|
50.00%
|
| Primer 23 | Rhizobium leguminosarum | AACGGCTTGCCATCGAGA | ATGCAGCGTCAGGTTCGT | 59.34 | 59.66 | 227 | 43 |
100.00%
|
100.00%
|
| Primer 24 | Rhizobium leguminosarum | TGCGACCGAATTGGGTGT | AAAGCTCTTGGCCAGCGT | 59.57 | 59.89 | 154 | 43 |
100.00%
|
50.00%
|
| Primer 25 | Rhizobium leguminosarum | TGCGACCGAATTGGGTGT | TTCCACGCTCTCGGCAAT | 59.57 | 59.34 | 178 | 43 |
100.00%
|
50.00%
|
| Primer 26 | Rhizobium leguminosarum | ACGCTGGCCAAGAGCTTT | CGATGACGGTGCTATCGCT | 59.89 | 60.01 | 259 | 43 |
100.00%
|
100.00%
|
| Primer 27 | Rhizobium leguminosarum | ACGCTGGCCAAGAGCTTT | ACGGTGCTATCGCTGAAGG | 59.89 | 59.86 | 254 | 43 |
100.00%
|
100.00%
|
| Primer 28 | Rhizobium leguminosarum | TGGGCATCGAAAGCTGGA | AACTGACGAACCAGCGCT | 58.92 | 59.58 | 198 | 43 |
100.00%
|
100.00%
|
| Primer 29 | Rhizobium leguminosarum | ACGGTTCGATGCGCTGAA | TCGATGCCCTTTTCCGCA | 60.05 | 59.65 | 177 | 36 |
100.00%
|
100.00%
|
| Primer 30 | Rhizobium leguminosarum | ACATTCCCAACCAGCGCA | GACTTCACCACCACGTCGT | 59.88 | 59.93 | 232 | 35 |
100.00%
|
50.00%
|